Re: Accutase for detachment for cell monolayers

Dear Giovanna,

Detaching adherent cells is not an exact science because we are dealing with
a biological system that is not always predictable because of unknown
variables.  We at ICT have discovered for instance that certain cell lines
when they are at a low passage number will lift off immediately with
Accutase, but at a high passage number are difficult to lift off and more
Accutase is required.

In addition, Accutase will not lift off all known cell lines as trypsin will
not.  I would recommend you try our other product for cell detachment and
tissue dissociation, Accumax, which has a higher concentration of enzymes in
it.  If this does not work another interesting phenomena we have just
discovered, is if enough EDTA is added to the Accumax (which contains none)
to give a .5mM concentration, the activity of the enzymes can be potentiated
by up to a factor of 3.

Please let me know if we can be of any more assistance.

C. Kevin Becker
Innovative Cell Technologies, Inc.
6790 Top Gun St. Suite 1
San Diego, CA 92121 USA
858 587-1716
Fax 858 453-2117
www.innovativecelltech.com
No pigs in our products!-Accutase/Accumax


----- Original Message -----
From: "Giovanna Farruggia" <giovanna.farruggia@unibo.it>
To: cyto-inbox
Sent: Monday, November 08, 2004 8:20 AM
Subject: accutase for detachment for cell monolayers


> Dear Flo,
> we are trying to use accutase to detach caco cells from very confluent
> monolayers to perform immunofluorescence assays in flow cytometry, and we
> have a lot of problem, lot of cluster and debris and it was very difficult
> to find scatter signals. Does anybody has experience with this product and
> some suggestions? Thank you a lot
>
> Giovanna Farruggia
> Dip. Biochimica "G. Moruzzi"
> Universita' di Bologna
> Via S. Donato 19/2
> 40127 Bologna
> tel. 051 2095626
> fax 051 2095627
>
>