analysis of mixed primary cultures of neurons

Has anyone ever tried to analyze a mixed population of neuronal cells 
from rat embryo primary culture.  I have been trying, unsuccessfully, 
to count a small population (up to 10%)  of neurons that are tyrosine 
hydroxylase positive from a larger mixed population of cells that 
includes glia, and neurons that are and are not tyrosine hydroxylase 
positive.  My strategy has been to label intracellular antigens that 
correspond to neurons (MAP2) and tyrosine hydroxylase.	I found only 
one reference that was specific for analyzing mixed neuronal cultures.	
If anyone has any experience analyzing neuronal cultures I would 
greatly appreciate your input.	Any general comments would also be 
welcomed.  Thanks in advance for your help.

Jeremy


Jeremy Schieler
Purdue University
Department of Medicinal Chemistry and Molecular Pharmacology
575 Stadium Mall Drive
Room 510
West Lafayette, IN 47907
(765) 494 6564