Re: Immunoflourescent Staining Help Needed

I recommend you look into the work of Rick Bucala.  He has published 12 
articles over the past 10 years on cells he refers to as "fibrocytes" - 
fascinating stuff.  Rick is almost as enthusiastic about fibrocytes as 
he is about MIF, which he has coauthored more than 100 publications over 
the same time period.  
The fibrocyte story is gaining some momentum now.  I have great 
admiration for Rick becauses he continues to publish on these cells in 
the face of great opposition from the proponents of dogma that 
frequently dismiss his work as artifact.  These cells don't quite fit 
into the current paradigms of cells found in peripheral blood.	I am 
curious about why you refer to your cells as stem cells - by what 
definition?

Best regards, Tom Delohery
I think our new official name is Sanofi-Aventis.... not quite sure which 
group I'll end up in;
hoping for global head of flow cytometry.  Every pharma needs one don't 
you think?

Brian Newsom wrote:

>I am staining a novel stem cell that is adherent and has morphology similar
>to a fibroblast. I am staining a mixture of extracellular and intracellular
>antigens but only one antigen/well and with FITC, I will have filter cubes
>for Cy-5 and others soon. Most antibodies are Mouse IgG and my secondary is
>a Donkey and mouse Ig FITC. Hopefully this will clarify any ambiguity in my
>initial query.
>
>Thanks,
>Brian
>  
>