Dear all, We have been asked to help on a diagnostic workout of a suspicious pulmonary Langerhans cell granulomatosis. Does anybody know whether flow analysis of bronchoalveolar lavage (BAL) would be of any utility? I was thinking on a combo of CD1a, HLA DR, CD11b and CD45, but can't find any clue of expected results, specially concerning FSC/SSC caractheristics of putative langerhans cells (I suppose a large number of epithelial cells will be present in the sample). I'm not familiar with flow analysis of BAL and any comment/suggestion would be very helpful. Should I filter the sample before staining? Thank you for your time! Marcelo Marcelo de Carvalho, MD PhD Virology-Immunology Lab BP 632 CHU Fort de France 97261 Fort de France Cedex Martinique, FWIReceived on Tue Oct 26 14:18:00 2004
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