Re: DNA content of nuclei from dried plant tissue

Dear Paul,

I recommend contacting Jan Suda suda@natur.cuni.cz (Botanical Institute of
the Charles Univesity in Prague) who has been quite succesful in using
herbarium vouchers.

Best regards, Jaroslav

**************************************************************
Dr. Jaroslav Dolezel
Laboratory of Molecular Cytogenetics and Cytometry
Institute of Experimental Botany
Sokolovska 6
CZ-77200 Olomouc
Czech Republic

Tel.: (+420) 585 228 521
Fax: (+420) 585 228 523
Email: dolezel@ueb.cas.cz
Web: www.ueb.cas.cz/olomouc1
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----- Original Message ----- 
From: "Paul Kron" <pkron@uoguelph.ca>
To: cyto-inbox
Sent: Thursday, October 21, 2004 9:43 PM
Subject: DNA content of nuclei from dried plant tissue


> Hi.
>
> I've been using flow cytometry for some time now to estimate DNA
> content of plant nuclei, usually for the purpose of determining ploidy.
> I have had good success with fresh, frozen (-80 degrees) and desiccated
> tissue, where tissue is desiccated either using silica desiccant or by
> simple air drying (eg. pressed in a book). Recently I have been trying
> to use leaf tissue from herbarium sheets, with little success. Does
> anyone have experience with using this kind of tissue, or knowledge of
> relevant literature?
>
> Thank you,
> Paul Kron
> pkron@uoguelph.ca
>