Dear Colleagues, I'm using the cell alkaline dissociation at my lab to calculate micronuclei in the rat liver after PHx (see samples in attached files). This method requires only Soerensen phosphate buffer and KOH or NaOH that we use with the same result (see I.V. Uryvaeva et al. An improved method of mouse liver micronucleus analysis: an application to age-related genetic alteration and polyploidy study. Mut.Res., 334 (1995), 71-80). I'm going to modify this method for chromosome aberration analysis by placing a fresh isolated tissue from colchicinated animal into hypotonic solution with further fixation. Did somebody meet this method before? What do you think about propriety of this method using for chromosome aberration? Thank you very much, Denis V. Guryev, Ph.D. Department of Radioecology, Institute of Biology, 28, Kommunisticheskaya St., Syktyvkar, 167982, Russia Office: +7-8212-436-301 Fax: +7-8212-240-163 E-mail: guryev@ib.komisc.ru This attachment - '2004_02_25@13_07_50.jpg' - 55.21 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/8753cb2c06d7756b9703c4c37de831b0a9071547.jpg This attachment - '2004_02_25@12_58_02.jpg' - 58.51 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/a4aa550519faae022d12813642586cd6d152edf5.jpgReceived on Thu Oct 21 15:18:00 2004
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