Hi Gayle, One can use several agents to induce apoptosis in human PBMNC. A simple method we use that works well is with H2O2, read out as Annexin V/PI on flow. You get a nice dose response after culturing freshly isolated PBMNC for 18 hours in 0.05-10 mM H2O2. The data can be expressed as an apoptotic index (early apoptotic AnnV+PI- and late apoptotic AnnV+PI+). Hint: we find best results with H2O 2 bought at our corner drugstore, don't bother with high grade stuff. A Disclaimer: The above terminology does not take into account complexities about whether or not Annexin V expression distinguishes apoptosis from necrosis. I'm not sure if there is additional complexity to your query? Regards, Stan. ------------------ Gayle Buller wrote: > Does anyone have experience working with peripheral lymphocytes and > trying to induce apoptosis? Your experience would be quite helpful. > Thanks > Gayle BullerReceived on Tue Oct 19 15:58:00 2004
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