One way to induce apoptosis of lymphocytes (from peripheral blood) is to mitogenically stimulate them e.g. with polyvalent mitogen phytohemagglutinin (PHA). While large fraction of lymphocytes undergoes mitogenic stimulatiom, about 10-15% of lymphocytes undergoes so called "activation induced apoptosis". In fact, 48 h after addition of PHA variety of cells at different stages of mitogenic stimulation (G1, S, G2/M, also nonstimulated G0 cells) as well as apoptotic cells are present in cultures. 24 h after addition of PHA there are predominantly G1, G0 and apoptotic cells. The link below is to our recent paper on this topic 147.52.72.117/IJO/2002/volume21/number6/1245-1250.pdf Zbigniew Darzynkiewicz -----Original Message-----From: Gayle Buller [mailto:gayle.buller@Probes.com] Sent: Friday, October 15, 2004 2:36 PM To: cyto-inbox Subject: Lymphocyte Apoptosis Induction Does anyone have experience working with peripheral lymphocytes and trying to induce apoptosis? Your experience would be quite helpful. Thanks Gayle BullerReceived on Tue Oct 19 15:38:00 2004
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