RE: Do your have any DIVA problems?

That sounds like a plan.

Customer 'our cytometer that you built isn't working right with our experiments"

Vendor	'your experiments aren't working right with OUR cytometer.'

Customer  'hmmmm.... what to do?   '

Vendor 'change your experiments!'

Customer  'or I could change vendors'

sounds simple enough to me.

Chris




--Original Message Text---
From: Akos Szilvasi
Date: Fri, 15 Oct 2004 10:12:39 -0400


I pasted below a response to our Diva problems.  

In short there are some serious limitations "built in" to Diva that renders the LSR II to
a less than perfect cytometer. 100,000 
event limit is unacceptable for any cytometer. Especially not for one that can collect
over a dozen parameters. If you apply 
complex gating to your analyses, run an Indo-1 Ca++ flux experiment or go after rare
events you may need more than one or 
two hundred thousand cells collected. The recommended 50 tube/experiment is a new feature
I have not seen in the color 
catalog I received. I guess the delay of the HTS's release for LSR II is due to a
shortage of 50 well Falcon plates.  

 What is the solution? We are very interested in BD's official response. We are about to
buy three more of the new BD 
cytometers.  

Akos  

..............................................................  

As a general rule, when collecting 10 parameters per Tube, approximately 50
Tubes per Experiment can be recorded with 100,000 events per Tube.
Collecting more parameters (such as Area and Height) or having multiple
gates decreases the number of Tubes to 25 or fewer per Experiment. To save
memory (and disk space for permanent storage), save only parameters that
are being used.

. once they notice that Diva is slowing down,
that's a sign that their open Experiment is getting too large and they
should split it up into smaller experiments.  They should also check the
size of their database and clean it out on a regular basis.