RE: Do your have any DIVA problems?

We have a LSR DIVA 4.0 inst.  We currently have no problems with our unit.  However we
did have some.
I make sure that the Database is not larger than 3 GB.	And preferably less than 2 GB.	I
rarely defrag the drive.  I do not install any other software that may use Java except
for IE. If you ask the software to remember keystrokes by hitting aquire or record to
fast then you will have problems.  Just slow down a bit.  It is not a MAC so be gentle
with it.

Our PC is running NT Professional.  I wonder if those with recurrent problems are running
XP Prof?

Jim Houston

> ----------
> From: 	Akos Szilvasi
> Sent: 	Thursday, October 14, 2004 8:47 AM
> To:	Cytometry Mailing List
> Subject:	RE: Do your have any DIVA problems?
> 
> 
> Dear Diva Users, 
> 
> Thank you for the feedback. I am glad to see that our problems are not (entirely)
common with other machines. 
> 
> While everybody agreed that Java is a poor platform for a sophisticated and complex
acquisition software, most of you are satisfied with Diva. As I mentioned earlier, we are
enthusiastic about Diva's features. Only the Java-originated problems are overwhelming at
this time. Unfortunately BD's customer service and field service does not seem to be
skilled enough on this application to give us any advice beyond reinstalling the software
(which now I can do in my dreams) and defragmenting the HD (which gets 80% fragmented
after one hour of operation). 
> 
> The main issue is that when we are running larger volume of multicolor (7 or 8 color)
experiments after 30-40 samples the saving process slows down to 35-45 seconds/sample and
eventually different Java error messages pop up. Something like "Java generated too many
errors". Then we have to reboot the hardware. Often multiple times in order to re-load
the Java machine. This way a 150 sample run takes a full day and a half. The computer has
nothing on it except for some flow data (not a lot) and the database is being kept lean.
Java is the one that came with the Diva 4 disk. We are not upgrading Java. 
> 
> I very much hope we only have some sort of setting problem and it will be solved soon.
We are on the market for several new BD cytometers and want to get an idea of what we are
up to. I only wish BD would consider eliminating the Java from underneath Diva. 
> 
> Thanks for your feedback. Keep me updated if you have any suggestions. 
> 
> Akos 
> 
> 
> ...........................................
> Akos Szilvasi
> Biogen Idec Flow Cytometry
> 12 Cambridge Center, 
> Cambridge, MA 02142
> tel  617 679 2160
> fax 617 679 2304
> akos.szilvasi@biogenidec.com 
> 
> 
> 
> "Perfetto, Steve (NIH/VRC)" <perfetto@mail.nih.gov> 
> 
> 13-Oct-2004 07:33 PM To
> 'Akos Szilvasi' <Akos.Szilvasi@biogenidec.com> 
> cc
> Subject
> RE: Do your have any DIVA problems?
> 
>	
> 
> 
> 
> 
> Akos,
> 
> Many of these overflow errors are due to an incorrectly set FSC "o" bar or
> threshold setting. The "noise" counts can quickly over power the rate
> counter in the firmware and lock up the sytem.In addition, it is absolutely
> necessary to defrag your disk routinely with frequent deletion of data. We
> routinely download all of our files to a terabyte server and delete files
> from the database weekly. We currently have two 20 parameter LSR II's, one
> DiVA and one Aria, with this strategy we have avoided many of these issues.
> I agree that JAVA is probably not ideal for the type of software but at this
> point that is the structure we must work within.
> 
> Stephen P. Perfetto, MS.,MT. (ASCP)
> Manager, Core Flow Cytometry Facility
> Vaccine Research Center, NIH
> 40 Convent Dr., Room 5507
> Bethesda, MD 20892-3015
> 
> email: sperfetto@nih.gov
> Phone: (301) 594-8659
> 
> > ----------
> > From:		   Akos Szilvasi
> > Sent:		   Wednesday, October 13, 2004 10:50 AM
> > To: 		 Cytometry Mailing List
> > Subject: >			Do your have any DIVA problems?
> > 
> > 
> > We are experiencing serious  - and so far not fixable - problems with DIVA
> > 4. Most of the issues are Java related. In addition error and overflow
> > messages combined with couple of other issues. We are planning to buy a
> > second LSR II and an ARIA but this Java based software is rather scary. 
> > 
> > I would like to see how many of you are experiencing some sort of
> > difficulties with Diva. We especially want to know about the Java errors
> > and stability issues. 
> > 
> > Thanks, Akos 
> > 
> > [An other note: before you buy a new cytometer consider future upgrades
> > and ask how much they cost. You may be surprised.] 
> > 
> > ...........................................
> > Akos Szilvasi
> > Biogen Idec Flow Cytometry
> > 12 Cambridge Center, 
> > Cambridge, MA 02142
> > tel  617 679 2160
> > fax 617 679 2304
> > akos.szilvasi@biogenidec.com
> > 
> 
> 
>