anti-mouse FasL antibody

Dear all,

I am having difficulty staining for FasL on mouse T cells and need
helpful suggestions. The clone I am using from Pharmingen is MFL3
(PE-anti mouse FasL, cat. #555293) that is not supposed to be strain
specific. I tried to use it stain anti-CD3 activated T cells for 40
minutes on ice but I cannot get any staining. Does anyone used it before
and have a good method and suggestions?

 

Thank you very much

 

Yan Su

 

PhD Candidate

Immunology Program

The George Washington University