Question about FACS on primary human solid tumour cells

To whom it may concern, 
I recently started trying to do flowcytometry on primary human colon cancer
cells. I obtain the tissue and then generate a single cell suspension. My
viability prior to fax is apprx.50%-60% (tyrpan blue). However, my facs data
is showing viability in the  1-2%? I was wondering if anyone has any
recommendations or advice for me on isolating primary human colon cancer
cells and carrying out facs on these cells ? 
Thank you, 
Catherine O'Brien 
University of Toronto 


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