FW: about the marker for RAG -/- mice

From: Gerstein, Rachel <Rachel.Gerstein@umassmed.edu>
Date: Fri Oct 01 2004 - 13:53:24 EST
> ----------
> From: 	Gerstein, Rachel
> Sent: 	Friday, October 1, 2004 2:45 PM
> To:	'Li  Li'
> Subject:	RE: about the marker for RAG -/- mice
> 
> Hi
> 
> if you take PBL and analyze live cells using PI to exclude dead cells, you can easily
see the lack of lymphocytes in rag KO's using just a scatter gate (FSC vs SSC).
> 
> good luck,
> Rachel
> 
> =======================================================
> Rachel M. Gerstein, Ph.D.
> Department of Molecular Genetics and Microbiology
> Graduate Program in Immunology/Virology
> University of Massachusetts Medical School
> 55 Lake Avenue North
> Worcester, MA 01655-0002
> (508) 856-1044
> (508) 856-5920 (FAX)
> 
> 
> ----------
> From: 	Li  Li
> Sent: 	Wednesday, September 29, 2004 6:54 PM
> To:	Cytometry Mailing List
> Subject:	about the marker for RAG -/- mice
> 
> hi, guys,
>	We would like to use flow to do the genotyping for 
> the RAG 1-/- mice. Who know which marker is good  for this 
> and also RAG 2-/-? Thanks.
> 
> Li Li
> Research Associate,
> INM-Nephrology,
> University of Virginia
> 
> 
> 
Received on Mon Oct 4 13:58:00 2004

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