Bessie, CD4CD8 double positives can be a FACS artifact, but they can also represent "real" lymphocytes, namely antigen-specific memory cells, and can be cloned at 1 cell per well in vitro (proving that they are not aggregates of single cells). The percentage and activation status of these cells increases in response to infections as we have recently described in humans and in an animal model of hepatitis C virus infection. <<Nascimbeni, Blood.pdf>> Regards, Barbara Rehermann, MD Liver Diseases Section, NIDDK National Institutes of Health Bldg 10, Room 9B16 10 Center Drive Bethesda, MD 20892-1800 phone: (301) 402-7144 Fax: (301) 402-0491 Email: Rehermann@nih.gov > ---------- > From: Bessie Kam > Sent: Monday, September 27, 2004 5:13 PM > To: Cytometry Mailing List > Subject: CD4+//CD8+ in apparently normal individual > > Hello, > I would like to ask your opinion on the following individual. > We have a middle-aged male with a WBC count of 6.0 and 42% > lymphocytes. Repeat one month later showed a similar WBC and drop of > lymphocytes to 19%. The individual is not clinically symptomatic. > > On both occasions the individual showed that 20% of lymphocytes coexpress > CD4 and CD8. These coexpressing lymphs were CD2+, CD3+ (72%) and CD5+ and > > lacked CD7. TdT and CD56 were also negative. 8% of lymphocytes were CD19 > > positive with polyclonal kappa/lambda expression. What do you think this > > means? > > thanks for your help > > -- > Bessie Kam Email: bkam@dls.queens.org > The Queen's Medical Center, Pathology FAX: 808-547-4045 > Phone: 808-547-4271 > > This attachment - 'Nascimbeni, Blood.pdf' - 632.12 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/7a1aa720711e713bd8602dfcc7ad87f46187e1e4.pdfReceived on Wed Sep 29 13:58:00 2004
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