Hi Eric, I think if you are doing PI as exclusion dye for live/dead cells, a 488 laser should work. I have used 488 laser in Annexin V-FITC/PI assay where PI is used to identify dead cells where apoptotic cells are tracked by Annexin V-FITC. The assay seems to work reasonable well. Hope this help. Albert Exelixis Inc. -----Original Message----- From: ERICA LEIGH BEHLING-KELLY [mailto:behlingkelly@wisc.edu] Sent: Friday, September 24, 2004 6:48 AM To: cyto-inbox Subject: PI at 488? Hello all, I seem to be getting some contradicting info re. propidium iodide staining for live/dead differentition and the ability to do so without having a UV laser. Judging from the excitation spectra, it looks like you can get some excitation with a 488 laser. I am wondering if this is enough to allow for discrimination of live dead cells without having to use an inappropriately high gain. I only have access to a 488 laser and would like to run assays using PI to gate out dead cells and FITC conjugated antibodies for my antigen detection So, does anyone out there run live/dead assays using PI and only a 488 laser? Thanks in advance, Erica Erica Behling-Kelly, DVM Department of Pathobiological Sciences University of Wisconsin 2015 West Linden Drive Madison, WI 53706 behlingkelly@wisc.eduReceived on Mon Sep 27 14:58:00 2004
This archive was generated by hypermail 2.1.8 : Tue Sep 28 2004 - 03:12:07 EST
