surface and cytoplasmic antigen staining

Dear Flowers,

I have a question about surface versus cytoplasmic antigen staining. For
example, speaking of kappa/lambda, when we do not see anything on the
surface, we still have a chance to find light chain restriction by doing
cytoplasmic staining. But when we identify antigens on the cell surface,
does this automatically means, that these antigens are to be found inside
the cells? In other words, when we go straight for intracellular
kappa/lambda expression (like in myeloma cases), do we still consider
surface staining of any value?

Thank you all. Irina

Irina Grigorieva, PhD
Director, Flow Cytometry Laboratory
Northside Hospital, Atlanta, GA
(404)- 851-6541
e-mail: irina.grigorieva@northside.com







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