Hi FLOWers, I have a researcher who would like to sort live cells (for subsequent culture) based on the expression of estrogen receptor expressed on the nuclear membrane. Has anyone done anything similar?? If so could you please pass along any tips on how to prepare the cells and if I need to treat them any differently to my standard sorts to ensure viability? I use a BD FACSVantage and usually sort around 14psi & 38000Hz. Many thanks Kathy -- Dr Kathryn Heel Biological Imaging and Analysis Facility The University of Western Australia M510 Level 1, M Block, QEII Medical Centre Nedlands WA 6009 Ph +61 8 9346 4525 Fx +61 8 9346 3469 Mb +61 0 416 085 960Received on Fri Sep 17 11:27:54 2004
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