Cox-1 & Cox-2

Hi Everyone,

I am in the early stages of setting up a Cox-1&2 flow assay in human whole 
blood monocytes for our laboratory.
The assay will be used to monitor the in vitro inhibitory effects (if any) 
of certain extracts on both Cox-1 & 2.
I am using LPS to stimulate Cox-2 levels, with unstimulated monocytes going 
from approx. 3% to 95% Cox-2 positive (post stimulation)
However LPS appears to have little if any effect on Cox-1 levels with both 
pre & post stimulation levels being around the 1% mark.
It would therefore be very difficult to assess any inhibitory agent effect 
on Cox-1 at those levels.
Does anyone know of a Cox-1 stimulatory agent that I could incorporate into 
the assay, so that I can monitor any inhibitory effects on Cox-1 as well as 
Cox-2 ?
Any thoughts or comments would be really appreciated.

Thanks
Paul
-----------------------------------------
Paul Connellan
Research Associate	
Natural Products Pharmacology Unit
(Joint Venture ACCMER/CPP)
Centre for Phytochemistry & Pharmacology,
T Block,
Southern Cross University,
Lismore NSW 2480

Phone: +61 02 66223211
Fax:	 +61 02 66223459

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