Re: Displaying Indo-1 data

FlowJo has successfully worked for users here to display the mean of 
the ratio over time.  It allows you to generate a time parameter 
(order of collection) or will use an instrument generated time 
parameter. You can derive your own ratio or use the instrument ratio 
parameter. There are some other nice touches as well, including 
movies.

Marty

Marty Bigos
Director, Flow Cytometry Core Laboratory
Gladstone Institute of Virology and Immunology

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At 2:14 PM +0100 09/02/04, Matthew Henry wrote:
>Hi,
>
>I'm attempting to measure intracellular calcium fluxes using Indo-1 
>on a MoFlo and I have a question regarding displaying my data.
>
>So far I have presented my data as a dotplot of time (X axis) vs 
>FL5/FL6 (y axis). This shows the increase in intracellular calcium 
>over time, but as it is a dotplot its quite 'messy'.
>
>Is there a way/program etc that can take the mean 'dot density' and 
>display it as a line, thus producing a nice line graph of time vs 
>FL5/FL6? I have seen papers which have displayed data in this format 
>but they don't say how they did this. Can this be done in WinMDI, 
>FloJo, Cell Quest Pro, FCS Express???
>
>I hope this makes sense.
>
>Thanks
>
>Matthew Henry
>
>CD45 Group
>
>The Edward Jenner Institute for Vaccine Research
>
>Newbury
>
>UK
>
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