RE: Displaying Indo-1 data

 Hi Matthew,

One word-FlowJo.  Use the kinetics platform-works great for Fluo-3 data and
I am sure it will work well for indo.  When in doubt-call TreeStar-always
very helpful to me.  By the way, I have no financial or other interests in
FlowJo-like the majority of us, when I find something that works for my
research, I try to get the word out to help all my colleagues.

Good luck,

Randy T. Fischer
NIAMS/NIH


-----Original Message-----
From: Matthew Henry
To: cyto-inbox
Sent: 9/2/2004 9:14 AM
Subject: Displaying Indo-1 data

Hi,

I'm attempting to measure intracellular calcium fluxes using Indo-1 on a
MoFlo and I have a question regarding displaying my data.

So far I have presented my data as a dotplot of time (X axis) vs FL5/FL6
(y axis). This shows the increase in intracellular calcium over time,
but as it is a dotplot its quite 'messy'. 

Is there a way/program etc that can take the mean 'dot density' and
display it as a line, thus producing a nice line graph of time vs
FL5/FL6? I have seen papers which have displayed data in this format but
they don't say how they did this. Can this be done in WinMDI, FloJo,
Cell Quest Pro, FCS Express???

I hope this makes sense.

Thanks

Matthew Henry

CD45 Group

The Edward Jenner Institute for Vaccine Research

Newbury

UK

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