Here is a reference of a very good protocol on platelet activation. I have been using this method oover the years and it is very simple. Platelet surface P-selectin measurements in platelet preparations: an international collaborative study. Biomedical Excellence for Safer Transfusion (BEST) Working Party of the International Society of Blood Transfusion (ISBT). Transfus Med Rev. 1999 Jan;13(1):31-42. " Rudensky" <rudensky@szmc.o To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> rg.il> cc: Subject: Activation of platelets 08/22/2004 02:48 AM We would like to introduce some research in the study of activation of platelets using CD62P as the activation marker. I would greatly appreciate receiving a detailed protocol from anyone actually performing these assays in their laboratory, with perhaps some unwritten tips, as I understand that the assay can be tricky. Thank you all in advance. Dr. Bernard Rudensky Clinical Laboratory Director Shaare Zedek Medical Center P.O. Box 3235 Jerusalem, Israel 91031 Fax +972 2 6555857 ********************************************************************** This message was checked by McAfee Antivirus !!! No viruses found ********************************************************************** Shaare Zedek Medical Center - http://www.szmc.org.ilReceived on Tue Aug 24 14:38:00 2004
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