Dear Barbara: Please see the following reference that may help with the identification and sorting of microglia: Andrew L. Ford, Anna L. Goodsall, William Hickey, Jonathon D. Sedgewick Normal Adult Ramified Microglia Separated from Other Central Nervous System Macrophages by Flow Cytometric Sorting Journal of Immunology, 1995, 154:4309-4321. Good luck, Andy Morschauser Schering-Plough Research Institute -----Original Message----- From: Barbara Oppenlander [mailto:oppenb@msn.com] Sent: Saturday, August 14, 2004 11:37 AM To: cyto-inbox Subject: Sort mouse brain cells Dear flowers: We wish to sort mouse and/or rat brain cells into their major populations: neurons, astrocytes, microglia, etc. Is anyone aware of a published methodology which would help in this endeavor? Medline searches for specific surface markers for those populations have not yet yielded much useful information. Thanks a lot for your help. Barbara Oppenlander ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete.Received on Wed Aug 18 14:58:00 2004
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