Re: ethidium monoazide and annexinV

Molecular Probes has 4 "reduced biohazard" viability kits that label dead cells
(488/green,
488/red, UV/blue options) covelently and stand up to fixation. They are all set up as
incubate / (fix) / run. The red emitting version (cat # L23102) should be essentially a
drop-in.

Regards,
Bill Godfrey
Molecular Probes



"Alice L. Givan" wrote:

> Hello Flowers,
> I am trying to advise someone about doing an annexin-V apoptosis assay in conjunction
> with cytoplasmic staining for cmyc.  The problem is in the inability to identify
> dead/necrotic cells by PI or 7-AAD (after the cells have been fixed for the cmyc
> staining).
>
> Has anyone tried doing the annexin-V assay in conjunction with ethidium monoazide
instead
> of PI?  My idea would be to stain with annexin-V,  then photochemically bind the
ethidium
> monoazide to the dead cells,	then  fix the cells and stain for cmyc.  How does that
> sound?
>
> Alice
>
> Alice L. Givan, Director
> Englert Cell Analysis Laboratory
> of the Norris Cotton Cancer Center
> Dartmouth Medical School
> Lebanon, NH 03756 USA
> tel 603-650-7661
> fax 603-6540-6130
> givan@dartmouth.edu
> www.dartmouth.edu/~celllab