Ikke sensitiv 110804 (subheading necessary due to security) Dear flowers! Sometimes after ac-lysing the white cells seem to suffer and when doing flow cytometry we see a bunch of cells from the negative quadrant heading towards upper right. There is obviously unspecific staining and the cells are probably dead or dying. The phenomenon may be present when lysing whole blood or bone marrow - most often it happens to one of the samples from the patient, not with both even when being prepared at the same time. We have checked pH in the lysing solution and the fixative, carefully checked the lysing time, tried to vary temperature in the solutions we are using, and tried to avoid using 2% PFA as the last step in the staining procedure. Anyone else having the problem or the solution to this problem? Regards Sylvi Johansen Medical Technologist Dep. of Immunology and Transfusion Medicine University Hospital of Northern Norway Tromsų NorwayReceived on Wed Aug 11 13:38:00 2004
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