Re: exporting raw data from gated events

Actually, you can save a text file directly from a gated (or ungated)
dotplot in WinMDI. File -> Save As -> then select Tabbed Text from the file
type list box, next type in the new file name under File Name, and then
click on Save. Under Preferences you can preselect if the text is the
channel value or the arithmetic value for log parameters.

      Joe


		      Gerald Gregori							  		      <gregori@flowcyt.cyto.	     To:      Cytometry Mailing List
<cytometry@flowcyt.cyto.purdue.edu>			     
		      purdue.edu>		     cc:      (bcc: Joe Trotter/SDCA/BDX) 						     Subject: Re: exporting raw data from
gated events						
		      07/29/2004 10:52 AM						  



Hi Chris and Janet,

here is a possible solution with WinMDI. It is a little more tricky than
other software (like WinLIST or FCS Express) but it is free and use WinMDI.
Draw your region in your dor plot, then gate this region only on your
dot plot in order to display only the cells you want. Now go to
File/Save as/ and save your file still in FCS format but with a
different name. WinMDI will save only the particles displayed on your
plot in the gate.

Now, use LLDATA (link :
http://www.cyto.purdue.edu/flowcyt/software/ldatapp.htm) which is a
freeware we developped here in Purdue to convert FCS file into a text
file (.txt). Because LLDATA can convert a bunch of files, the most time
consuming part is the gating and saving in WinMDI.

I hope it helps.
Gerald

 :
janet dow wrote:

> Dear Flowers:
>
> I am writing this email for a client -here is his question in his words:
>
> I am interested in doing some analysis beyond that of the software we
> have available.  In order to do this, I need to be able to export raw
> data (individual light scatter and fluorescent values for each event)
> from a gated region.	       The gate needs to be a polygon rather than a

> simple square.    Currently we have CellQuest, WinMDI, and Summit
> software.  I am most familiar with CellQuest and WinMDI, but I have
> not found a way to export data.  I have just started to use Summit
> software and have not determined yet whether it is capable to export
> data.  I have also tried MFI (PC-Dos), but this software is limited to
> square gated regions.
>
> My question is how can I export the raw data within a gated region?
> Will either CellQuest, WinMDI, or Summit have the capacity to do
> this?  If not, what software should I use?  We also have access to
> FlowJo software.
>
> I would appretiate any assistence or specific instructions you may
> have.  I can be reached at chris@genejockeys.com and I will also try
> to monitor any responses through Janet Dow whom is forwarding this
> message.
>
> Thank you for your help.
> Chris Halweg
> North Carolina State University


--

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Dr. GÉRALD GRÉGORI

Purdue University Cytometry Laboratories

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West Lafayette, IN 47907-2064,USA

Ph : +1(765) 494-0757
Fax: +1(765) 494-0517

e-mail: gregori@flowcyt.cyto.purdue.edu

http://www.cyto.purdue.edu
http://www.cyto.purdue.edu/gregori/cyto_web/indexang.html

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