A note of caution, if you plan to publish data selectively cleaned by this type of approaches, you should better state in the figure legend and in your material and methods that the data shown only represent the gated events but in not way represent the bulk of the whole population. Wisely, you should show the bulk data and the gated used. Otherwise, it could be interpreted by your reviewers and the readers as intentional hiding of undesirable information. Regards Rafael>===== Original Message From Gerald Gregori <gregori@flowcyt.cyto.purdue.edu> ===== >Hi Chris and Janet, > >here is a possible solution with WinMDI. It is a little more tricky than >other software (like WinLIST or FCS Express) but it is free and use WinMDI. >Draw your region in your dor plot, then gate this region only on your >dot plot in order to display only the cells you want. Now go to >File/Save as/ and save your file still in FCS format but with a >different name. WinMDI will save only the particles displayed on your >plot in the gate. > >Now, use LLDATA (link : >http://www.cyto.purdue.edu/flowcyt/software/ldatapp.htm) which is a >freeware we developped here in Purdue to convert FCS file into a text >file (.txt). Because LLDATA can convert a bunch of files, the most time >consuming part is the gating and saving in WinMDI. > >I hope it helps. >Gerald > > : >janet dow wrote: > >> Dear Flowers: >> >> I am writing this email for a client -here is his question in his words: >> >> I am interested in doing some analysis beyond that of the software we >> have available. In order to do this, I need to be able to export raw >> data (individual light scatter and fluorescent values for each event) >> from a gated region. The gate needs to be a polygon rather than a >> simple square. Currently we have CellQuest, WinMDI, and Summit >> software. I am most familiar with CellQuest and WinMDI, but I have >> not found a way to export data. I have just started to use Summit >> software and have not determined yet whether it is capable to export >> data. I have also tried MFI (PC-Dos), but this software is limited to >> square gated regions. >> >> My question is how can I export the raw data within a gated region? >> Will either CellQuest, WinMDI, or Summit have the capacity to do >> this? If not, what software should I use? We also have access to >> FlowJo software. >> >> I would appretiate any assistence or specific instructions you may >> have. I can be reached at chris@genejockeys.com and I will also try >> to monitor any responses through Janet Dow whom is forwarding this >> message. >> >> Thank you for your help. >> Chris Halweg >> North Carolina State University > > >-- > >--------------------------------------------- >--------------------------------------------- > >Dr. GRALD GRGORI > >Purdue University Cytometry Laboratories > >Hansen Life Sciences Research Building, B050 >201 S. University Street, >West Lafayette, IN 47907-2064,USA > >Ph : +1(765) 494-0757 >Fax: +1(765) 494-0517 > >e-mail: gregori@flowcyt.cyto.purdue.edu > >http://www.cyto.purdue.edu >http://www.cyto.purdue.edu/gregori/cyto_web/indexang.html > >--------------------------------------------- >--------------------------------------------- Dr. Rafael Nunez MD. Assistant Professor in Medicine-Hematology/Oncology and Pathology Director UIC-Cancer Center Flow Cytometry Core Facility Hematology-Oncology Section, Department of Medicine Department of Pathology University of Illinois at Chicago MBRB, Room 3211/3168, M/C 734 900 South Ashland Ave. Chicago, IL 60607 Phone: 312 3551448 and 312 3550123 Fax: 312 4137963 Email: rafaelnr@uic.eduReceived on Tue Aug 3 15:18:00 2004
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