Dear Flowers: I am writing this email for a client -here is his question in his words: I am interested in doing some analysis beyond that of the software we have available. In order to do this, I need to be able to export raw data (individual light scatter and fluorescent values for each event) from a gated region. The gate needs to be a polygon rather than a simple square. Currently we have CellQuest, WinMDI, and Summit software. I am most familiar with CellQuest and WinMDI, but I have not found a way to export data. I have just started to use Summit software and have not determined yet whether it is capable to export data. I have also tried MFI (PC-Dos), but this software is limited to square gated regions. My question is how can I export the raw data within a gated region? Will either CellQuest, WinMDI, or Summit have the capacity to do this? If not, what software should I use? We also have access to FlowJo software. I would appretiate any assistence or specific instructions you may have. I can be reached at chris@genejockeys.com and I will also try to monitor any responses through Janet Dow whom is forwarding this message. Thank you for your help. Chris Halweg North Carolina State University -- Janet Dow Laboratory Research Specialist and Manager Flow Cytometry and Cell Sorting Facility North Carolina State College of Veterinary Medicine Room C-309 Raleigh, NC 27606 (919)513-6443Received on Thu Jul 29 11:58:00 2004
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