Fellow flowers, I have a colleague that needs a questioned answered. He is using dihydrorhodamine 123 to measure ROS in yeast. He is running his samples in triplicate. However, he is getting 3 different fluorescence intensities from these samples. One would expect that these samples would be relatively the same. We first thought that it might be an equipment issue, but I am skeptical. My experience with this dye is very limited. Does anyone with more experience than myself have an idea about what is going on here? Thanks in advance for your help, Mike ******************************** Michael Hulsey, MS Technical Director, Flow Cytometry Emory Vaccine Center Emory University Atlanta, GA 30329 404-727-8619 mhulsey@rmy.emory.edu ********************************Received on Thu Jul 15 12:38:00 2004
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