Re: Memory T- cell marker.

Hi there,

I know it has been a long tine to reply, but I have been away for a while.

For memory T cells, it depends on which species you are interested in. 
In humans, the most commonly used marker is CD45RO, which you can 
combine with CD4 to get CD4+/CD45RO+ memory T helper cells or with CD8 
to get memory T cytotoxic cells CD8+/CD45RO+. Some also simply do 
CD3+/CD45RO+ when if is not useful to differentiate between helper and 
cytotoxic T cells.

In rats, most people use CD45RC (memory cells are CD45RC- in that case). 
Memory t helper cells would be CD4+/CD45RC-. I don't have the reference 
on top of my head right now, but I also remember an article where they 
discriminating memory helper T cells from recent thymic emigrants using 
CD90, and "true" memory helper T cells were CD4+/CD45RC-/CD90-.

I am presenting a poster on rat memory T and B cells at the FOCIS 2004 
meeting here in Montreal. If you happen to be there, I would be happy to 
give you more details

Martin

-- 
Martin Poirier
Research Scientist, Immunology
CTBR Bio-Research Inc.

On 2004-03-15 07:48, Subramanian Manogaran wrote:

>Hi flowers,
>
>Someone here  wants to study memory T cells using Flow cytometry . Could someone ,
>please suggest me a best CD marker or combination of markers that can be  used to
>identify  these memory T cells ?  
>Thanks in advance .
>
>P.S.Manogaran.
>Flow cytometry core facility
>Research Centre, MBC #03
>KFSH&RC
>P.O.BOX 3354
>Riyadh-11211
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>
>Phone: 00 966 1 4647272  ext  32860
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