Hi Karen, I have no experience with your current experiment but I do have a potential solution. qdot.com is selling a Qtracker kit with up to 3 colors 565, 655 and 705nm. If you excite with the 407 laser and use either the 655nm or 705nm dots, you should get the data you are looking for with minimal spectral overlap. Good luck. Glenn ************************************************* Glenn Paradis MIT Flow Cytometry Core Facility E18-463 Massachusetts Institute of Technology Cambridge, MA 02139 (617)253-6454 lab (617)452-4132 office (617)253-3714 fax gap@mit.edu http://web.mit.edu/flowcytometry/www/ ************************************************* At 2:05 PM -0400 6/29/04, Karen Creswell wrote: >Does anyone have experience using a cell tracker dye (to track multiple >populations) along with calcium measurements using fluo-3/FuraRed? We >have a FACSAria with 3 lasers (407, 488, and 633). > >Thanks for your help! >Karen > >-- >Karen Creswell, Ph.D. >Director, Flow Cytometry/Cell Sorting Shared Resource >Lombardi Cancer Center >Georgetown University Medical Center >Research Bldg W304 >Box 571469 >Washington, DC 20057-1469 >202-687-2233 >creswelk@georgetown.edu --Received on Thu Jul 1 13:38:00 2004
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