Hi, Lately, I have been facsing many rabbit polyclonal antibodies. I have been using Molecular Probes goat anti-rabbit IgG Alexa Fluor 488 and Jackson Immunoresearch donkey F(ab')2 anti-rabbit IgG (H+L) FITC or APC as secondary reagents. I use fluorochromes that don't overlap with propidium iodide because I like using PI as a marker of cell death (yeah, I know PI is more a marker of membrane permeability, so humor me a bit here and I do know that APC and PI overlap in their emission wavelengths). Problems I have run into are these secondary reagents are not bright at all, the rabbit IgG preps tend to have a lot of non-specific binding and purified rabbit IgG is a lousy control. I have also tried the Molecular Probes rabbit Zenon kit and the results were less than satisfactory. Anyone have any suggestions? Richard C. Hastings Associate Scientist Target Development/TBD 1800 Concord Pike Wilmington, DE 19803 302-886-2452 richard.hastings@astrazeneca.comReceived on Wed Jun 16 13:38:00 2004
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