no, you cannot fix them. the dye leaks into all the cells if they are fixed. ron On Jun 8, 2004, at 1:52 PM, Melendez, Johana wrote: > Hello ! > I will like to know if JC-1 stained cells can be fix for later > acquisition. I read a lot of literature and could not find anything > about this regard. My guess it's no, but I'm not sure. If anybody > can answer this question for me I'll appreciate it. > Thanks > Johana > > Johana Melendez, MS > Research Associate > Flow Cytometry Laboratory > H. Lee Moffitt Cancer Center > 12902 Magnolia Dr. > Tampa, Florida 33612-9497 > Phone (813) 745-6610 > Lab. phone (813) 972-8400 x. 2005 > Fax (813) 903-7140 > <<Melendez, Johana.vcf>> > > ###################################################################### > This transmission may be confidential or protected from disclosure and > is only for review and use by the intended recipient. Access by > anyone else is unauthorized. Any unauthorized reader is hereby > notified that any review, use, dissemination, disclosure or copying of > this information, or any act or omission taken in reliance on it, is > prohibited and may be unlawful. If you received this transmission in > error, please notify the sender immediately. Thank you. > > ###################################################################### > <Melendez, Johana.vcf> Ronald L. Rabin, M.D. Senior Staff Fellow Laboratory of Immunobiochemistry DBPAP/OVRR Center for Biologics Evaluation and Research U.S. Food and Drug Administration 29 Lincoln Drive (MSC-4555) Building 29, Room 129 Bethesda, MD 20892-4555 phone: 301.496.8806 fax: 301.402.5177 email: rr84g@nih.govReceived on Thu Jun 10 14:58:00 2004
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