Dear Manuel: I've been using the BD Biosciences CBA kits for quite some time and have been very pleased with the overall ease of running samples and analyzing the data on the CBA software. I have been using the CBA Human TH1/TH2 Kit II [IL-2,4,6,10, TNFa, IFNg] kit for the past six months to evaluate activated T cell cytokine production and was extremely pleased with the sensitivity and reproducibility from assay to assay and kit to kit. Contact me directly if you need further information. Hope this helps. Best regards, -Rich Richard F. Konz, Jr. Senior Scientist Novartis Institutes for BioMedical Research, Inc. HT Flow Cytometry Facility Functional Genomics 100 Technology Square Cambridge, MA 02139 Office: 617-871-3238 Lab: 617-871-7339 Fax: 617-871-7053 email: richard.konz@pharma.novartis.com Manuel Comabella Lopez <mcomabel@vhebron.net> 06/08/2004 10:12 AM To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> cc: Subject: CBA versus flowcytomix Dear Flowers, We are highly interested in setting up the determination of several cytokines in supernatants from cell cultures. We are awared of two commercially available flow cytometry based kits that work on a similar way: the CBA from Becton Dickinson, and the Flowcytomix from Bender MedSystems (Innogenetics). Could anyone enlighten us about which one works better in terms of user-friendliness, sensitivity, and reproducibility? Best regards, Manuel Comabella Clinical Neuroimmunology Unit Vall Hebron Hospital Barcelona SpainReceived on Wed Jun 9 15:18:00 2004
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