Hello Horst, Neutrophils from patient with Specific granules deficiency (SGD) or Chediak Higashi Syndrome (CHS) behave the same as normal neutrophils (they can be a little bit bigger). We isolated the cells with percoll (density=1.076) or ficoll (1.077) gradients (You can always checked the layer on top of this gradient for the presents of neutrophils). We lysed the cells with ammoniumchloride and they survived this treatment. The experience we have with Dextran Radioselectan gradients where not promising. Erik Mul, ing Sanquin Research at CLB Dept. of KVI Plesmanlaan 125 1066 CX AMSTERDAM The Netherlands phone: +31-205123435 fax: +31-205123310 email: e.mul@sanquin.nlReceived on Thu Jun 3 13:38:00 2004
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