Dear collegues! Is there anybody who has ever isolated granulocytes from a patient with Specific granue deficiency (SGD) or Chediak Higashi Syndrome (CHS) ? If so: How did you prepare the cells of the patients with SGD and CHS for FACS analysis ? Do you have any experience with Dextran Radioselectan gradients or other gradients for the isolation of granulocytes in these patients ? Have you performed a lysis of erythrocytes prior to FACS analysis ? I am a little concerned whether the Dextran RAdioselectan gradient will work in the usual way in SGD and CHS and whether lysis may harm granulocytes of these patients quicker than those of healthy controls... Any word of advice would be strongly appreciated... Thank you in advance Horst Horst von Bernuth, MD Laboratoire de Génétique Humaine des Maladies Infectieuses Unité Mixte de Recherche Université René Descartes-INSERM U550 Faculté de Médecine Necker-Enfants Malades 156 rue de Vaugirard 75015 Paris, France, UE Tel 01 40 61 53 07 Copie 01 40 61 56 88 E-mail vonbern@necker.fr -------------------------------------------------------------- Horst von Bernuth, MD Laboratory of Human Genetics of Infectious Diseases University of Paris René Descartes-INSERM U550 Necker-Enfants Malades Medical School 156 rue de Vaugirard 75015 Paris, France, EU Voice 33 1 40 61 53 07 Fax 33 1 40 61 56 88 E-mail vonbern@necker.frReceived on Tue Jun 1 12:58:00 2004
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