Feedback on Cyflow Green Partec

Dear flower,

My name Polidy Pean, Researcher Assistant at Pasteur Institute of Cambodian.
I have a question concerning the CyflowGreen Partec.

I have experience with FACScalibur BD.
Since a year, I have been appointed to supervise  Cyflow Green Partec, 
which had been installed in an Hospital of Phnom Penh Capital of Cambodia 
last year, for measuring CD4 counting (Absolute Count ) for HIV infected 
patients.

Even there are many evidences indicate that the absolute number of CD4 
generated by Cyflow Green Partec highly correlated with CD4 absolute 
counting generated by FACScaliburBD ( TruCount Multiset) and FACScount BD, 
but pratically I encountered many problem concerning this Instruments.

I would like to inform you that for the Cyflow Green Partec in Cambodia, We 
perform the CD4 aboslute counting by using only mono labeled ( CD4PE) and 
then we analyzed by gating on CD4 bright in dot plot parameters ( CD4 PE vs 
SSC).

The problem is the gating strategy-that mean we couldn't see the cluster of 
CD4 bright in dot plot.

I accept every suggestion and comment about the advantages and disavantages 
of this Instruments .
Also, I would like to have a precise report and feed back of this 
Instrument too.

Thank you very much in advance

POLIDY PEAN