At 04:21 PM 5/25/2004, Wolfraim, Lawrence (NIH/NCI) wrote: >What type of cells and how many did you transfer? None yet! The transfer is today of B220+ splenocytes from an immunized mouse into a SCID. I am hoping for 2e7 cells. >The extend of dilution of CFSE (i.e., proliferation) depends entirely upon >what cells you are transferring (T cells?, other) and into what host Yup, that's the big question. I don't know how many divisions they will undergo. Anyway, this is just a pilot since I can't sort at the moment... just about the entire flow world is off in France! Depending how this goes, I'll repeat it later on sorted (rather than dynel-selected) populations. But I think the easiest course is to do leave FL2 empty for the time being and use PerCP and APC with the CFSE cells. I am a little concerned that if I use freshly labeled cells they will be unmanageably bright... Thanks, Jeff -- Jeffrey Rice <rice@aecom.yu.edu> Diamond Lab Microbiology and Immunology/Sue Golding Graduate Division Albert Einstein College of Medicine, Bronx, NY 10461 [voice] 718-430-4112 [fax] 718-430-8711Received on Wed May 26 13:38:00 2004
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