Fwd: Re: neutrophil elastase

DMSO also causes differentiation into neutrophils

George

attached mail follows:

Mary, retinoic acid terminally differentiates HL-60 to neutrophils.  
TPA "makes them" into macrophages, and I believe a relatively high pH 
"makes them" into eosinophils.	Don't know what DMSO does.

ron

On May 20, 2004, at 1:44 PM, Stetler-Stevenson, Maryalice (NIH/NCI) 
wrote:

> This isn't a flow question but I bet a flower knows the answer. 
> Someone in
> our group is in need of cDNA from neutrophils to clone out neutrophil
> elastase. They have ancient HL60s in the freezer and need to find out 
> if it
> is TPA or DMSO to differentiate them. Think this will work? Any better
> ideas?
>
> Maryalice Stetler-Stevenson
> Chief, Flow Cytometry Unit
> Laboratory of Pathology, NCI, NIH
>
> Sometimes you're the windshield, sometimes you're the bug.
> <mime-attachment>
Ronald L. Rabin, M.D.
Senior Staff Fellow
Laboratory of Immunobiochemistry
DBPAP/OVRR
Center for Biologics Evaluation and Research
U.S. Food and Drug Administration
29 Lincoln Drive (MSC-4555)
Building 29, Room 129
Bethesda, MD   20892-4555

phone:	301.496.8806
fax:	301.402.5177
email:	rr84g@nih.gov