Mary, retinoic acid terminally differentiates HL-60 to neutrophils. TPA "makes them" into macrophages, and I believe a relatively high pH "makes them" into eosinophils. Don't know what DMSO does. ron On May 20, 2004, at 1:44 PM, Stetler-Stevenson, Maryalice (NIH/NCI) wrote: > This isn't a flow question but I bet a flower knows the answer. > Someone in > our group is in need of cDNA from neutrophils to clone out neutrophil > elastase. They have ancient HL60s in the freezer and need to find out > if it > is TPA or DMSO to differentiate them. Think this will work? Any better > ideas? > > Maryalice Stetler-Stevenson > Chief, Flow Cytometry Unit > Laboratory of Pathology, NCI, NIH > > Sometimes you're the windshield, sometimes you're the bug. > <mime-attachment> Ronald L. Rabin, M.D. Senior Staff Fellow Laboratory of Immunobiochemistry DBPAP/OVRR Center for Biologics Evaluation and Research U.S. Food and Drug Administration 29 Lincoln Drive (MSC-4555) Building 29, Room 129 Bethesda, MD 20892-4555 phone: 301.496.8806 fax: 301.402.5177 email: rr84g@nih.govReceived on Mon May 24 12:58:00 2004
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