ISAC XXII Workshop 3 Wednesday, May 26, 2004 1400 - 1600 Cell Sorting for Microgenomics Co-Chairs: James F. Leary and Peter Szaniszlo Gene expression microarray profiles represent the weighted average of all of the different cell types present. Contaminating cell types can have major or minor effects on specific genes depending on the expression levels in the cells of interest and the contaminating cells. Cell separation by flow cytometry/cell sorting, magnetic sorting, laser-ablation technologies and other technologies can have a major effect on the overall gene expression profile. This is also affected by the time needed to perform the cell separation since some of the RNA may degrade rapidly. What degrees of purity are required? How can the gene expression profile be "frozen" in time to permit reasonable analyses? When only small numbers of cells (< 50,000 cells) are available, linear amplification methods need to be used to amplify all of the mRNA to obtain enough material for spotted or oligo microarray analyses. The theoretical and practical problems, and promise, of such amplification technologies for "microgenomics" will be discussed in this workshop based on three years' experience of the workshop leaders.Received on Fri May 21 11:58:00 2004
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