Re: Experiences with Cytek Time zero system for Calcium flux

Alex, I used the Cytek time Zero system for our studies of calcium flux 
in response to chemokines (Rabin et al J Immunol 162;3840; 1999).  In 
general, we were pretty happy with it.	Its value depends upon the sort 
of responses you are trying to detect.	If they are rapid, transient 
responses on small populations of cells (e.g. chemokines on 
lymphocytes) then Time Zero is the way to go because you might miss the 
response without it, or accept an artifactual signal from boosting as 
real.  If you are looking at more prolonged and high amplitude 
responses that have a reasonable delay time (e.g. anti-CD3), then you 
really don't need it.

ron

Ronald L. Rabin, M.D.
Center for Biologics Evaluation and Research
USFDA
Bethesda, MD   20892
Phone:	301.496.8806
FAX:	  301.402.5177
On May 12, 2004, at 7:36 AM, Alexander Schmitz wrote:

> Hi Flowers,
>
> One of my "clients" is interested in doing CalciumFlux experiments 
> (with Indo-1)on our FACS-VantageSE.
> I would like to hear about your experiences with Cyteks TimeZero/ 
> TimeWindow systems.
> Simply speaking: Is it worth buying ?
>
> Any Comments are welcome
> Alex
>
> PS: I also contacted Cytek directly today.
>
>
>
>
>
> ______________________________________
> Dr. Alexander Schmitz
> FACS Core Facility
> Department for Molecular Biology (MBI)
> C.F. Moellers Allee 130/3
> DK 8000 Århus
> Denmark
> axs@mb.au.dk
>
> ______________________________________
>
>
>
>