Hello Jamie, Check the reference below. The rats we used for the paper below were of various ages. Steve Goldman has published extensively on the differentiation of neuronal, glia and other cells isolated from brain tissue from multiple sources since the paper below was published. writing from home, currently at Aventis Pharm, TomD Wang S; Wu H; Jiang J; Delohery TM; Isdell F; Goldman SA Isolation of neuronal precursors by sorting embryonic forebrain transfected with GFP regulated by the T alpha 1 tubulin promoter [published erratum appears in Nat Biotechnol 1998 May;16(5):478] Nat Biotechnol 1998 Feb;16(2):196-201 ----------------------------------------------------------------------------------------- ---- Jamie Lynn Peters wrote: >Hello everyone! > >Does anyone know if flow cytometry can be used on adult rat brain tissue? I am >trying to separate some fluorogold labeled cells from all other cells in a >specific neuroanatomical region I have dissected from adult rat brain, and I'm >wondering if it is possible to use flow cytometry to accomplish this. My only >other option is performing laser capture microdissection, and I should mention >that I intend to extract RNA from these cells for RT-PCR. I have not been able >to find any references of using this technique with adult rat brain tissue. >This may be because connections are dense, and there is a great deal of >myelination in the adult brain, making it difficult to dissociate the cells. >However, if anyone knows of a way to make this feasible, I would be forever >indebted to you for your advice! Please direct any comments to >petersjl@musc.edu. Thank you! > >Sincerely, > >Jamie Peters > > >Received on Mon May 10 13:18:00 2004
This archive was generated by hypermail 2.1.8 : Tue May 11 2004 - 03:12:04 EST
