See http://cyto.mednet.ucla.edu/7aad%20Staining%20for%20dead%20cells.htm We use this protocol and it works well. Joanne Lannigan, MS Director, Flow Cytometry Core Facility University of Virginia Jordan Hall, Room 7067 P.O. Box 800734 Charlottesville, VA 22908-0734 Office: 434-924-0274 Lab: 434-243-2695 Fax: 434-982-1071 email: joannelannigan@virginia.edu -----Original Message----- From: Stetler-Stevenson, Maryalice (NIH/NCI) [mailto:stetler@mail.nih.gov] Sent: Tuesday, May 04, 2004 9:10 AM To: cyto-inbox Subject: 7AAD Does anyone have a good protocol for using 7AAD for viability, followed by wash and fix? We need to fix cells for next day runs and need to do viability. Thanks. Maryalice Stetler-Stevenson Chief, Flow Cytometry Unit Laboratory of Pathology, NCI, NIH Sometimes you're the windshield, sometimes you're the bug.
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