Hello Karim, I'd use PI, My guess is that you are not interested in the non-viable cells anyway and the PI -ve population shouldn't affect your SNARF staining. I use PI as a viability stain in conjunction with PE/PerCP etc with no problems Best regards, Arnold >Hello, >What's the best way to check viability of SNARF-1-labeled cells by flow on a single-laser system? As far as I understand, SNARF-1 emits in the same wavelength as 7-AAD, and possibly overlaps with PI as well. >If the only solution to this problem involves a 633 nm laser, what would you suggest as an optimal viability/dead cell marker? >Thanks for any info! >Karim > > >Karim Vermaelen, MD, PhD > >NASA Johnson Space Center >Building 37 - Room 1096 >2101 NASA Parkway >77058 Houston, TX >USA > >tel +281 244 1993 >fax +281 483 3058 > > _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 020-7679-6234 Fax: +44 020-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/Received on Mon May 3 13:18:00 2004
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