Optiprep reduction of contaminating platelets

From: bunny <bunny@cotleur.com>
Date: Thu Apr 22 2004 - 14:23:19 EST
(Belated) Happy Easter!

Does anyone have experience with Otiprep (from Axis-Shield) as a way to 
reduce platelets in PBMC prep's?
I would love to hear how well it removes the plt's (when compared to 
slow washes with EDTA and such), and any useful hints/tips you found.
thanks!

bunny


Bunny Cotleur, M.S.
Sr Research Technologist, Neurosciences
Scientific Consultant, Flow Cytometry Core
Cleveland Clinic Foundation
Lerner Research Institute, NC30
9500 Euclid Avenue
Cleveland, OH 44195
Lab: (216)444-1164
*********************************************
When you do something, you should burn yourself completely, like a good 
bonfire, leaving no trace of yourself.
(Shunryu Suzuki)
Received on Fri Apr 23 16:58:00 2004

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