RE: Biosafety of sorting revisited

From: Stephen Sullivan <sjs93@cam.ac.uk>
Date: Thu Apr 15 2004 - 08:50:49 EST
Hi all, I am sorting a very small population of cells (about 500) for 
someone in my lab. She wants to generate RNA from the sorted cells. Does 
anyone have any technical advice regarding generating RNA from a very small 
population of sorted cells....what kit did you use? Also what settings 
should you use to increase the concentration of sorted cells (i.e. minimise 
the sheath fluid accompanying positive sorted cells in the side stream)? 
Thanks Stephen
Received on Fri Apr 16 13:38:00 2004

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