In my opinion the original article in the NEJM had ambiguous flow data and if you look closely the gates are "floating". I'm not surprised. The molecular data, however, were cleaner. -----Original Message----- From: Suter, Mike [mailto:MSuter@omlabs.com] Sent: Tuesday, April 06, 2004 7:42 PM To: cyto-inbox Subject: ZAP-70 vs. CLL Greetings, We have run about 2 dozen CLL cases against ZAP-70 from various vendors. Gating on T cells from a normal blood shows good positivity with antibody from all vendors. The CLL lymphocytes have been either negative or dimly positive compared to T cells, however. I haven't been able to find any clearly positive cases. This runs counter to the idea that ZAP-70 expression works as a surrogate marker for unmutated IgVH gene status, which occurs in roughly half of CLL patients. I will append a couple of histograms showing a case with very slight B-cell positivity, and would appreciate any technical suggestions. We are running on a Beckman Coulter XL, staining cells after permiabilization with Coulter IntraPrep reagent. Is anyone getting unambiguous results with ZAP-70 testing by flow? Thanks, Michael Suter Oregon Medical Labs *** HUMC's Proactive Security and Virus Scanner has scanned this email for malicious content and it is safe to use*** ***************************************************************************************** ********* Important news about our email communications. Hackensack University Medical Center has implemented secure messaging services. If you need assistance with retrieving a secure email, please send an e-mail to postmaster@humed.com ***************************************************************************************** ********** Confidentiality Notice: This e-mail message and any attachments from Hackensack University Medical Center are confidential and for the sole use of the intended recipient. This communication may contain Protected Health Information ("PHI"). PHI is confidential information that may only be used or disclosed in accordance with applicable law. There are penalties under the law for the improper use or further disclosure of PHI. If you are not the intended recipient of this e-mail or the employee or agent responsible for delivering the communication to the intended recipient, then you may not read, copy, distribute or otherwise use or disclose the information contained in this message. If you received this message in error, please notify us by telephone at 201.996.2000 or by e-mail to postmaster@humed.com. Please indicate that you were not the intended recipient, and confirm that you have deleted the original message. Please do not retransmit the contents of the message. Thank you. Hackensack University Medical Center is the proud recipient of Quality New Jersey's Governor's Gold Award for Performance Excellence ***************************************************************************************** *********Received on Thu Apr 8 13:18:01 2004
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