We have used the Lyse wash assistant for over a year and the SPA for almost a year. Never any problems with cell loss with the LWA. It's the flow operators' best friend. The SPA uses lots of reagents, you have to be careful and try to dilute antibodies to make it worthwhile. The SPA is a little slow, but it works really well with our biohazard samples. You can also fudge the software to omit the mixing step and use it with open tube samples. I would recommend both units. Ernest Stapleton Division Manager Immunology and Genetics Laboratories Room 1524 Health Sciences Center St. John's, Newfoundland A1B 3V6 Canada 709-777-8654 -----Original Message----- From: lawrence johnson [mailto:lawrencereveljohnson@msn.com] Sent: Saturday, April 03, 2004 12:15 PM To: cyto-inbox Subject: Sample Prep and Lyse Wash Assistant for BD FACSCalibur Hello, Has anyone used or had experience with the BD FACSCalibur Sample Prep unit? If so, have there been any significant problems, such as loss of sample, staining issues and the like? Also, any thoughts on the BD Lyse Wash Assistant? Many thanks, Lawrence Lawrence R. Johnson, MD University of Pittsburgh School of Medicine Division of HematopathologyReceived on Wed Apr 7 13:38:00 2004
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