Greetings, We have run about 2 dozen CLL cases against ZAP-70 from various vendors. Gating on T cells from a normal blood shows good positivity with antibody from all vendors. The CLL lymphocytes have been either negative or dimly positive compared to T cells, however. I haven't been able to find any clearly positive cases. This runs counter to the idea that ZAP-70 expression works as a surrogate marker for unmutated IgVH gene status, which occurs in roughly half of CLL patients. I will append a couple of histograms showing a case with very slight B-cell positivity, and would appreciate any technical suggestions. We are running on a Beckman Coulter XL, staining cells after permiabilization with Coulter IntraPrep reagent. Is anyone getting unambiguous results with ZAP-70 testing by flow? Thanks, Michael Suter Oregon Medical Labs <<ZAP70.PCX>> <<ZAP70CD1.PCX>> -------------------------------------------------------- This message is intended solely for the use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable state and federal laws. If you are not the addressee, or are not authorized to receive for the intended addressee, you are hereby notified that you may not use, copy, distribute, or disclose to anyone this message or the information contained herein. If you have received this message in error, immediately advise the sender by reply email and destroy this message. -------------------------------------------------------- This attachment - 'ZAP70.PCX' - 10.90 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/6e2ba311a331927a76b8da8b7ca7665a105b3633.PCX This attachment - 'ZAP70CD1.PCX' - 10.85 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/5c67bef078fc140d526fd8f21a39566e32123529.PCXReceived on Wed Apr 7 12:58:00 2004
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