I have a labmate who is exploring using SNARF-1 for proliferation studies in transgenic eGFP expressing mouse B lymphs. As I was looking at his initial data, I was hit by a thought. Since SNARF-1 is a sensitive pH indicator, and eGFP does have a pKa, will the intracellular pH differential between eGFP plus and minus cells confound proliferation algorithms? In other words, does the altered pH of eGFP+ cells change the MFI of the SNARF-1 enough to make it moot to compare eGFP+ to eGFP- populations, as he is wanting to do. I plan on suggesting a side-by-side staining of transgenic and WT mousey cells to directly measure the difference, but I also thought that asking the list was a fast and informative correlation. Thanks in advance! -CBReceived on Tue Mar 23 13:38:00 2004
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