Using SNARF-1 with eGFP

From: Cris Bare <flowmail@VERIZON.NET>
Date: Tue Mar 23 2004 - 11:15:34 EST
I have a labmate who is exploring using SNARF-1 for proliferation studies 
in transgenic eGFP expressing mouse B lymphs. As I was looking at his 
initial data, I was hit by a thought.

Since SNARF-1 is a sensitive pH indicator, and eGFP does have a pKa, will 
the intracellular pH differential between eGFP plus and minus cells 
confound proliferation algorithms?  In other words, does the altered pH of 
eGFP+ cells change the MFI of the SNARF-1 enough to make it moot to compare 
eGFP+ to eGFP- populations, as he is wanting to do.

I plan on suggesting a side-by-side staining of transgenic and WT mousey 
cells to directly measure the difference, but I also thought that asking 
the list was a fast and informative correlation.

Thanks in advance!

-CB
Received on Tue Mar 23 13:38:00 2004

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